Part:BBa_K2722004:Experience
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2722004
We used this part to create a composite part, which expresses this mTQ protein. This fluorescent protein was used to quality control the expression efficiency of our self-made cell extract in comparison to purchased cell extracts. Cell extract is a composition of all E.Coli plasma proteins, that has all the required machinery for transcription and translation processes outside of a living cell. This can be used for rapid characterisation of biorbicks, genetic circuits, expression of toxic proteins and a lot more.
We have measured mTQ fluorescence at different concentrations. Giving a linear concentration to fluorescence blot, which might be used to calculate concentrations from fluorescence.
The extinction coefficient ε is calculated with following formula: ε=A/(c ∙l)
Whereby A is the Absorption, c is the concentration in Mol and l is the pathlength of the probe. We measured the Absorption at 434 nm wavelength of six samples. Three having a concentration of 22 µM and another three having a concentration of 11 µM mTQ protein on a Nanophotometer (l = 1 mm). And calculated the extinction coefficient.
Absorption Extinction coefficient [1/Mcm]
Sample1: 22 µM;Absorption:0,068; Extinction coefficient: 30,909 1/Mcm
Sample2: 22 µM Absorption:0,076 Extinction coefficient:34,545 1/Mcm
Sample3: 22 µM Absorption:0,075 Extinction coefficient:34,091 1/Mcm
Sample4: 11 µM Absorption:0,031 Extinction coefficient:28,182 1/Mcm
Sample5: 11 µM Absorption:0,030 Extinction coefficient:27,273 1/Mcm
Sample6: 11 µM Absorption:0,030 Extinction coefficient:27,273 1/Mcm
Mean extinction coefficient value: 30,379 1/Mcm
We generated a mean value from our six samples of 30,379 1/Mcm. Which is consistent with the literature value.
User Reviews
UNIQ0766a81ec440f905-partinfo-00000000-QINU UNIQ0766a81ec440f905-partinfo-00000001-QINU